Saturday, July 31, 2021

LSU student identifies, names new soybean fungal pest

• By Bruce Schultz •

teddy garcia aroca
Teddy Garcia-Aroca, an LSU doctoral student, holds a sample of a fungus he found and named that causes the disease soybean taproot decline — photo by Bruce Schultz, LSU AgCenter

An Louisiana State University graduate student has identified and named a new fungal species that causes a devastating soybean disease, and the finding has been published in a scientific journal.

LSU doctoral student Teddy Garcia-Aroca identified and named the fungus Xylaria necrophora, the pathogen that causes soybean taproot decline. He chose the species name necrophora after the Latin form of the Greek word “nekros,” meaning “dead tissue,” and “-phorum,” a Greek suffix referring to a plant’s stalk.

“It’s certainly a great opportunity for a graduate student to work on describing a new species,” said Vinson Doyle, LSU AgCenter plant pathologist and co-adviser on the research project. “It opens up a ton of questions for us. This is just the tip of the iceberg.”

The Louisiana Soybean and Feed Grain Research and Promotion Board helped fund the project detailed in Garcia-Aroca’s paper.

Soybean taproot declin

The fungus infects soybean roots, causing the roots to become blackened and leaves to turn yellow or orange with chlorosis. The disease has the potential to kill the plant.

“It’s a big problem in the northeast part of the state,” said Trey Price, LSU AgCenter plant pathologist who is Garcia-Aroca’s major professor and co-adviser with Doyle.

“I’ve seen fields that suffered a 25% yield loss, and that’s a conservative estimate,” Price said.

According to the paper, Louisiana soybean losses from the disease totaled 1.2 million bushels in 2018.

Price said the disease has been a problem for many years as pathologists struggled to identify it. Some incorrectly attributed it to related soybean diseases such as black-root rot.

“People called it the mystery disease because we didn’t know what caused it,” he said.

Price said while Garcia-Aroca was working on the cause of taproot decline, so were labs at the University of Arkansas and Mississippi State University.

Price said the project is significant. “It’s exciting to work on something that is new. Not many have the opportunity to work on something unique.”

“It’s the only Xylaria species that causes disease on an annual crop in the world. Most Xylaria pathogens infect woody perennials,” Price said.

The paper was published in “Mycologia,” a journal of the Mycological Society of America, focusing on the biology of fungi emphasizing subjects such as molecular biology and plant pathology.

Examining samples

Garcia-Aroca compared samples of the fungus that he collected from infected soybeans in Louisiana, Arkansas, Tennessee, Mississippi and Alabama with samples from the LSU Herbarium and 28 samples from the U.S. National Fungus Collections that were collected as far back as the 1920s.

Some of these historical samples were collected in Louisiana sugarcane fields but were not documented as pathogenic to sugarcane. In addition, non-pathogenic samples from Martinique and Hawaii were used in the comparison, along with the genetic sequence of a sample from China.

Garcia-Aroca said these historical specimens were selected because scientists who made the earlier collections had classified many of the samples as the fungus Xylaria arbuscula that causes diseases on macadamia and apple trees, along with sugarcane in Indonesia. But could genetic testing of samples almost 100 years old be conducted?

“It turns out it was quite possible,” he said.

DNA sequencing showed a match for Xylaria necrophora for five of these historical, non-pathogenic samples — two from Louisiana, two from Florida and one from the island of Martinique in the Caribbean — as well as DNA sequences from the non-pathogenic specimen from China. All of these were consistently placed within the same group as the specimens causing taproot decline on soybeans.

Garcia-Aroca said Xylaria necrophora has been found on decaying cotton and corn plants, but he said it’s not known if the fungus could also infect live corn or cotton plants. His paper says the fungus can infest soybean root tissue, but it is not clear if a wound to the host is needed for the pathogen to infect. And it’s possible that a byproduct of the fungus’ feeding is actually what causes the foliar symptoms associated with the disease.

The mystery remains

He said a hypothesis that could explain the appearance of the pathogen in the region is that the fungus could have been in the soil before soybeans were grown, feeding on decaying wild plant material, and it eventually made the jump to live soybeans.

The paper poses the question of why the fungus, after living off dead woody plant tissue, started infecting live soybeans in recent years. “Events underlying the emergence of X. necrophora as a soybean pathogen remain a mystery,” the study concludes.

But the paper suggests that changes in the environment, new soybean genetics and changes in the fungal population may have resulted in the shift.

The lifespan of the fungus is not known, Garcia-Aroca said, but it thrives in warmer weather of at least 80 degrees Fahrenheit. Freezing weather may kill off some of the population, he said, but the fungus survives during the winter by living on buried soybean plant debris left over from harvest. It is likely that soybean seeds become infected with the fungus after coming in contact with infected soybean debris from previous crops. These hypotheses remain to be tested.

Many of the fungal samples were collected long before soybeans were a major U.S. crop, Doyle said. “The people who collected them probably thought they weren’t of much importance.”

Garcia-Aroca said this illustrates the importance of conducting scientific exploration and research as well as collecting samples from the wild. “You never know what effect these wild species have on the environment later on.”

He said the work provided a subject for his doctoral dissertation, and he learned how to conduct DNA sequencing.

What now after identification?

Now that the pathogen has been identified, Price said, management strategies need to be refined. Crop rotation and tillage can be used to reduce incidence as well as tolerant varieties.

“We’ve installed an annual field screening location at the Macon Ridge Research Station where we provide taproot decline rating information for soybean varieties,” Price said. “In-furrow and fungicide seed treatments may be a management option, and we have some promising data on some materials. However, some of the fungicides aren’t labeled, and we need more field data before we can recommend any.”

He said LSU, Mississippi State and University of Arkansas researchers are collaborating on this front.

Doyle said Garcia-Aroca proved his work ethic on this project. “It’s tedious work and just takes time. Teddy has turned out to be very meticulous and detailed.”

The final chapter in Garcia-Aroca’s doctoral dissertation, Doyle said, will be further research into the origins of this fungus and how it got to Louisiana.

Bruce Schultz is assistant communications specialist at the LSU AgCenter. He may be reached at BSchultz@agcenter.lsu.edu

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